A high-throughput screening method for endophytic bacteria with antagonistic activity against Magnaporthe oryzae in rice (Oryza sativa L.) seeds

Screening for microorganisms with antagonistic activity against crop pathogens is essential microbial antagonist preparation, disease control, and sustainable agricultural development. This study established a rapid, efficient, one-step high-throughput method screening endophytic bacteria displaying Magnaporthe oryzae (M. oryzae) ACCC36020 in rice seeds. The experimental results indicated that these could be directly screened from six sample groups using this method. technique can achieve simultaneous purification, identify antifungal seeds, save significant time.